The chief chemist associated with General Ludendorff who specializes in chemistry and poisons. On her role, Anaya said, "Well, it was a small role in this big ensemble, but it is an important character in the story. I'm going to be a big nightmare" for Wonder Woman and Steve Trevor. Describing her character, Anaya said, "Dr. Maru loves rage and enjoys people's pain. She's creating terrible weapons, and her purpose in life is to kill as many people as possible, and provoke as much pain as possible". She researched World War I and Fritz Haber, the scientist who created mustard gas, to prepare for the role. On the character's facial scars, Anaya stated, "I went to Patty Jenkins and asked, 'What happened to her?' And she said, 'She did it on purpose.' I was like, 'What? Patty, you're going further than I ever imagined.' She said, 'She wants to provoke painful suffering, so she tested her own gas on her own face. She wanted to know how deep this form of her gas would go, so she put it on her own face.' You can see half of her face is completely gone. This is the sadistic side of Dr. Maru". She also stated her character "is quite the opposite to the lead role of this movie, one of the strongest characters ever of DC comics, Wonder Woman. I can tell you that Doctor Poison is someone with a capacity to provoke so much pain." On Dr. Maru's relationship with General Ludendorff, Anaya said, "I think that they have a relationship based on loyalty. Ludendorff is a very tormented General that lacks self-confidence. That's why, in part, he takes these drugs that Dr. Poison gives him. They are from different worlds, but they complement each other".
Wonder Woman (vol. 1) #179 (Nov. 1969) showed Wonder Woman giving up her powers and returning her costume and title to her mother, in order to continue staying in Man's World. The reason behind this was that all the Amazons were shifting to another dimension, but Diana was unable to accompany them as she needed to stay behind to help Steve, who had been wrongly convicted. Thus, she no longer held the title of Wonder Woman and after meeting and training under a blind martial arts mentor I-Ching, Diana resumed crime fighting as the powerless Diana Prince. She ran a mod-boutique as a business and dressed in a series of jumpsuits while fighting crime. During this period, Samuel R. Delany took over scripting duties with issue #202. Delany was initially supposed to write a six-issue story arc, which would culminate in a battle over an abortion clinic, but Delany was removed reportedly due to criticism from Gloria Steinem, who, not knowing the content of the issues Delany was writing, was upset that Wonder Woman had lost her powers and was no longer wearing her traditional costume.
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The Amazon Queen of Themyscira and Diana's mother. After meeting the director for the role, Nielsen said, "Patty and I met in London, and we just hit it off from the get-go. We couldn't stop talking. What was supposed to be a one-hour meeting turned into a two-and-a-half-hour lunch and we just really got each other." She described Jenkins's directing style for the film as "She's also the kind of director that I really flourish under. She has very strong and particular and specific ideas about what it is she wants to say. She comes from a place of strength always. And so, when you are dealing with someone like that, you feel absolutely free to be vulnerable, to be creative, and I am a big researcher." On playing the character, Nielsen said, "It was a complete and utter pleasure and I absolutely loved every second of playing her." On her character being Diana's mother and Amazonian queen, Nielsen stated, "I'm queen and I'm preparing my child for a world that entails a lot of responsibility. So it was important to me to bring that into the character." She read The Amazons by Adrienne Mayor to familiarize herself with women warriors and said "I used what I learned in Mayor's book as a rallying cry for how I approached Hippolyta. And then, of course, what is a leader who is elected by her peers every year and has been doing this for a thousand years? That too was interesting to think about". Nielsen went through a workout regimen for the film, saying "I did six hours a day. You know, two hours of weight training, two hours of swords training, and then two hours of horseback riding".
Having learnt of Wonder Woman's inability to return to Themyscira, Doctor Veronica Cale, a wealthy and powerful woman, set into motion a plan to use Wonder Woman to find Themyscira. Years earlier, Cale's daughter Izzy had her soul stolen by the gods Phobos and Deimos. They told Veronica that they would only return her daughter to her if she helped them find Wonder Woman, and got the location of Themyscira from her. Seeing no other option, Cale had formed a team called Godwatch, dedicated to locating Diana and Themyscira.