In an October 25, 1940, interview with the Family Circle magazine, William Moulton Marston discussed the unfulfilled potential of the comic book medium. This article caught the attention of comics publisher Max Gaines, who hired Marston as an educational consultant for National Periodicals and All-American Publications, two of the companies that would merge to form DC Comics. At that time, Marston wanted to create his own new superhero; Marston's wife and fellow psychologist Elizabeth suggested to him that it should be a woman:
The Amazon Queen of Themyscira and Diana's mother. After meeting the director for the role, Nielsen said, "Patty and I met in London, and we just hit it off from the get-go. We couldn't stop talking. What was supposed to be a one-hour meeting turned into a two-and-a-half-hour lunch and we just really got each other." She described Jenkins's directing style for the film as "She's also the kind of director that I really flourish under. She has very strong and particular and specific ideas about what it is she wants to say. She comes from a place of strength always. And so, when you are dealing with someone like that, you feel absolutely free to be vulnerable, to be creative, and I am a big researcher." On playing the character, Nielsen said, "It was a complete and utter pleasure and I absolutely loved every second of playing her." On her character being Diana's mother and Amazonian queen, Nielsen stated, "I'm queen and I'm preparing my child for a world that entails a lot of responsibility. So it was important to me to bring that into the character." She read The Amazons by Adrienne Mayor to familiarize herself with women warriors and said "I used what I learned in Mayor's book as a rallying cry for how I approached Hippolyta. And then, of course, what is a leader who is elected by her peers every year and has been doing this for a thousand years? That too was interesting to think about". Nielsen went through a workout regimen for the film, saying "I did six hours a day. You know, two hours of weight training, two hours of swords training, and then two hours of horseback riding".
Suddenly, Zola and the others were attacked by Artemis, who was sent by Apollo to kill Zeke, Zola's son. While Zola and the others escaped with Zeke, Wonder Woman took on Artemis by herself. Diana defeated Artemis and went to protect Zola and the others, while Ares took the defeated Artemis back to Apollo. However, the First Born and Cassandra, two of Zeus' illegitimate children, attacked Zola. Wonder Woman, Lennox and Orion worked together to fight the First Born, but he proved to be stronger than they had anticipated. Orion's Astro Harness incapacitated Cassandra, and Orion opened a Boom Tube so that Wonder Woman and her allies could escape. The First Born attempted to pry the Boom Tube open, but Lennox sacrificed himself so that the others could escape safely. Wonder Woman and her allies arrived at the other side of the Boom Tube and were greeted by Highfather, who explained that they were on New Genesis.